DNA Polymerase θ Increases Mutational Rates in Mitochondrial DNA
نویسندگان
چکیده
منابع مشابه
Promiscuous DNA synthesis by human DNA polymerase θ
The biological role of human DNA polymerase θ (POLQ) is not yet clearly defined, but it has been proposed to participate in several cellular processes based on its translesion synthesis capabilities. POLQ is a low-fidelity polymerase capable of efficient bypass of blocking lesions such as abasic sites and thymine glycols as well as extension of mismatched primer termini. Here, we show that POLQ...
متن کاملThe Mutational Specificity of DNA Polymerase - @ during in Vitro DNA
The frequency and specificity of mutations produced in uitro by eucaryotic DNA polymerase-@ have been determined in a forward mutation assay using a 250base target sequence in M13mp2 DNA. Homogeneous DNA polymerase-@, isolated from four different sources, produces mutations at a frequency of 4-6%/ single round of gap-filling DNA synthesis. DNA sequence analyses of 460 independent mutants res...
متن کاملDNA Polymerase θ: A Unique Multifunctional End-Joining Machine
The gene encoding DNA polymerase θ (Polθ) was discovered over ten years ago as having a role in suppressing genome instability in mammalian cells. Studies have now clearly documented an essential function for this unique A-family polymerase in the double-strand break (DSB) repair pathway alternative end-joining (alt-EJ), also known as microhomology-mediated end-joining (MMEJ), in metazoans. Bio...
متن کاملPurification of a Mitochondrial DNA Polymerase
The mitochondrial DNA polymerase from Crithidia fasciculata has been purified to near homogeneity. SDS-PAGE analysis of the purified enzyme reveals a single polypeptide with a molecular weight of approximately 43,000. The protein is basic, with an isoelec; tric point between 7.6-8.0. Its Stokes radius of 22 A and its sedimentation coefficient of 4.1 S suggest a native molecular weight of 38,000...
متن کاملMitochondrial DNA Mutations, Pathogenicity and Inheritance
Mitochondria contain their own DNA (mtDNA), which codes for 13 proteins (all subunits of the respiratory chain complexes), 22 tRNAs and 2 rRNAs. Several mtDNA point mutations as well as deletions have been shown to be causative in well-defined mitochondrial disorders. A mixture of mutated and wild type mtDNA (heteroplasmy) is found in most of these disorders. Inheritance of mtDNA is maternal, a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2018
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.8b00072